Abstract
Understanding seed coat fragment (SCF) spiking results in advanced fiber information systems (AFIS) analysis of seed coat neps (SCN) in ginned cottons is confounded by the opening of entangled fibers in the instrument’s fiber individualizer. This may influence seed coat tissue fragmentation and recovery since a high degree of machine-fiber interaction is required to individualize entities for sensing. In this paper, slivers were pre-opened in the AFIS followed by manual cleaning, spiking, and AFIS analysis. A protocol was developed to spike the pre-opened slivers. The percent recovery of the spiked entity was dependent on the species and cultivar of the cotton used to prepare the slivers. The lowest recovery was with Pima fibers. Also, the recovery improved with the increase in length of the fibers biologically attached to the SCF surface. Delinted seed coat fragments produced the lowest recovery. Seed coat fragments carefully removed from ginned lint and added to the processed slivers gave the highest recovery. Averaged SCN recoveries from two AFIS units ranged from 26 to 100% (theoretical). These results helped to explain why the AFIS analysis of SCN counts in processed cotton is lower than by the microscopic analysis.
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