Functions and regulation of miR-10b in Uyger colorectal cancer metastasis

Abstract

Objective To investigate the role of miR-10b in metastasis of colon cancer of Uyger patients. Methods miR-10b expression was tested on CRC samples which were divided into metastatic groups and non-metastic groups of Uyger petients.The miR-10b-overexpressed or down-regulated SW620 cells were used to test the effects of miR-10b on invasion and migration of SW620 cells and to observe how miR-10b regulating KLF4. The miR-10b/klf4-overexpressed or down-regulated SW620 cells were used to test E-cadherin, cyclins D1 and p53 expression to test the involved mechanism in the regulatory function of miR-10b on metastasis and proliferation in CRC cells. Results miR-10b was over-expressed in metastatic CRC samples, compared with non-metastic ones of Uyger patients (t=-5.372, P<0.005). Inhibition of miR-10b in SW620 cells led to a notable reduction in invasion and migration assay compared with control cells (t=26.56, 10.40, P<0.05). We transfected miR-10b inhibitor into SW620 cells, an in crease of KLF4 mRNA level and protein level in SW620 cells was observed (t=3.78, P<0.05). miR-10b knockdown and KLF4 overexpression upregulated E-cadherin expression. Correlated with the modulated expression of miR-10b: the inhibition of miR-10b caused downegulation of cyclins D1 and p53, which were partly abrogated after co-transfecting with miR-10b and siRNA KLF4. Conclusions Expression of miR-10b is up-regulated in the metastasis CRC tissues and cells. miR-10b controls the metastasis and proliferation of CRC cells. miR-10b may exert its effect on metastasis and prolieration of CRC cells by regulating the expression of KLF4.miR-10b appears to modulate several independent signaling pathways that involved EMT, cell cycle and apoptosis. Key words: Colorectal neoplasms; Neoplasm metastasis; miR-10b; Uyger