Nicotinamide phosphoribosyl transferase promoting colorectal cancer invasion, metastasis and proliferation

Abstract

Objective To investigate whether nicotinamide phosphoribosyl transferase (Nampt) can promote migration of colorectal cancer cells and its effect on proliferation. Methods The Transwell assay was used to examine the effect of Nampt on migration of SW480 and HT-29 cells. Western blotting was used to detect the expression of matrix metalloproteinase (MMP)-9 in SW480 and HT-29 cells. The effects of Nampt on cell cycle and DNA synthesis were tested after Nampt overexpression in SW480 and HT29 cells. Results The exogenous Nampt could promote the migration of SW480 and HT-29 cells in a dose-dependent manner, and simultaneously increase the protein expression of MMP-9 level in SW480 cells. The overexpression of Nampt in SW480 and HT29 cells could promote the entry of cells into the S phase and proliferation in SW480 cells (Nampt group: 51.21±0.78; blank control group: 31.47±0.90; and negative control group: 32.35±1.15, t=4.566, P<0.05) and HT-29 cells (Nampt group: 53.40±1.39; blank control group: 35.71±1.28; and negative control group: 33.15±2.04, t=5.432, P<0.05). Conclusion Nampt can promote the migration and proliferation of colon cancer cells. Key words: Nicotinamide phosphoribosyl transferase; Colorectal cancer; Migration; Proliferation