Functional properties of mesenchymal multipotent stromal stem cells obtained from bone marrow of patients with liver cirrhosis

Abstract

Aim. To study of the functional properties of autologous mesenchymal multipotent stem cells in patients with cirrhosis of different etiologies. Methods. Studied were the functional and phenotypic properties of autologous mesenchymal stem cells derived from the bone marrow in 35 patients (26 men and 9 women) with cirrhosis of the liver at the age of 19-53 years. Among the patients studied viral etiology was diagnosed in 18 (51.4%), alcohol - in 13 (37.1%), autoimmune - in 4 (11.4%) patients with liver cirrhosis. Results. Autologous mesenchymal stem cells from all of the observed patients were able to divide. The highest yield and the doubling rate of cell populations were observed in patients with viral cirrhosis. Comparative morphometric study of primary cultures derived stem cells in patients with autoimmune and alcoholic hepatitis, showed the presence of relatively small cells (less than 20 microns), which make up about 30% of the total number of cells. At the same time, in bone marrow derived cell cultures of patients with viral etiology of the disease the number of small cells was 1.5 times greater which makes approximately 49% of the total cell population. The vast majority of cultured stem cells, regardless of the etiology of cirrhosis express specific for these cells «stromal» markers - CD73, CD90 and CD105. Autologous mesenchymal stem cells isolated from bone marrow of patients with alcoholic liver cirrhosis characterized by the most pronounced pro-inflammatory, immunoregulatory potential. Conclusion. Functional and phenotypic properties of autologous mesenchymal stem cells in various forms of cirrhosis are different which requires adequate cultivation and correction before their transplantation.