Abstract
Collagen is used for a variety of biomedical and pharmaceutical uses, such as osteoarthritis-related pain management, hypertension, tissue engineering, and human implants, and is generally derived from porcine or bovine. Collagen from these animals has limitations due to the risk of disease transmission and religious constraints. Therefore, this study investigated the extraction of collagen from catfish (Silurus triostegus) waste. Acid-solubilized collagen and pepsin-solubilized collagen were extracted from catfish skin, fin, head, bone, and muscle. SDS-PAGE patterns of the extracted collagen showed that the protein molecular weights ranged from 97 to 200 kDa and skin, bone, and fin collagen consisted of 2 distinct α chains, which is typical of type 1 collagen. The proximate composition (moisture, protein, fat, and ash) and yield of the obtained extracts were determined. Skin collagen extracts were selected for further investigation due to the high collagen yield. The effects of the pH and salt concentration on solubility, and the denaturation temperature, FTIR spectra, reverse-phase HPLC, and SEM analysis were investigated to characterize the collagen samples. Based on the characterization of catfish skin collagen, this waste material has potential for use in the pharmaceutical and food industries.
Highlights
Catfish is a popular food, on the continents of Africa and Asia
All these findings were lower than those reported by Slimane and Sadok [15], who found that the acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) yields of cartilaginous fish were 23.07% and 35.27% on a dry weight, respectively
This study investigated the extraction of collagen from catfish (Silurus triostegus) skin, fin, head, bone, and muscle using acetic acid (ASC) and pepsin with acetic acid (PSC) extraction methods
Summary
Catfish is a popular food, on the continents of Africa and Asia. Collagen is a very well-known protein in vertebrates and comprises 30 percent of the total protein [1]. Collagen has a wide variety of biomedical and pharmaceutical uses, such as osteoarthritis-related pain management, hypertension, tissue engineering, human implants, and angiogenic disease inhibition [1] It is used for drug delivery, skin replacements, expandable intra-arterial stents, and cell attachment substrates as a dermal filler [3]. Collagen from fish skin, bones, and fins has denaturation temperatures that are dependent on the indigenous water environment and are typically between. Fish processing discards generally include skin, bones, scales, and fins. Collagen extraction generally consists of preparation or washing, cleaning, and cutting of the fish samples. The extraction efficiency was determined, and skin collagen extracts were characterized with respect to the molecular weight via SDS-PAGE, with solubility as a function of the pH and salt concentration, denaturation temperature, FTIR spectra, reverse phase high-performance liquid chromatography (HPLC), and morphology by SEM
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