FUNCTIONAL PROPERTIES, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF ZATARIA MULTIFLORA ENCAPSULATED IN GELTIN NANOFILMS

Somayeh Jahani,Amin Shakiba,Mehdi Azami

doi:10.15414/jmbfs.2014.4.2.88-92

Abstract

Development of biodegradable and biocompatible films based on the proteinpolymer with strong antibacterial activities is gradually obtained extensive concern in the world. The aim of this study was to evaluate the antioxidant and antibacterial properties of gelatin nano films merged with different concentrations of Zataria multiflora essential oil. Gelatin films were prepared from gelatin solutions (10% w/v) containing Zataria multiflora essential oil [ZMO] (2, 4, 6 and 8% w/w), glycerol (25% w/w) as plasticizer, and glutaraldehyde (2% w/w) as cross-linker. The mechanical, water solubility, water swelling, water vapor permeability, antioxidant and antibacterial properties of the films were measured according to the American Society for Testing and Materials. Gelatin films exhibited good tensile strength and elongation at break, water solubility, swelling, and water vapor permeability. Incorporation of ZMO into the gelatin films caused a significant decrease in tensile strength and swelling, and a significant increase in elongation at break, water solubility, water vapor permeability and whiteness of the films. Gelatin films exhibited low antioxidant activity while gelatin films incorporated with ZMO exhibited excellent antioxidant properties. The films incorporated with ZMO also exhibited excellent antibacterial properties against both Gram-positive and Gram-negative bacteria. Our results suggest that the gelatin/ZMO films could be used as a very attractive alternative to traditional materials for different biomedical applications.

Highlights

Gelatin is a soluble protein obtained by partial hydrolysis of collagen, the main insoluble fibrous protein constituent on bones, cartilages and skin (Ktari et al, 2014)
In this study we examined potential of antioxidant/antibacterial activities of gelatin films incorporate with Zataria multiflora essential oil (ZMO) as a wound dressing was investigated
Incorporation of ZMO into gelatin films caused a significant decrease in tensile strength and increase in elongation at break (P < 0.05)

Summary

Introduction

Gelatin is a soluble protein obtained by partial hydrolysis of collagen, the main insoluble fibrous protein constituent on bones, cartilages and skin (Ktari et al, 2014). There is growing interest in using plant extracts as natural sources of antioxidant/antibacterial compounds in formulating gelatin films (Appendini and Hotchkiss, 2002; Gomez-Guillen et al, 2009; Hanusova et al, 2009; Lucera et al, 2012). In this context, plant essential oils and their main components are gaining a wide interest in health industry for their potentials as antioxidant and antimicrobial agents, since they are generally recognized as safe (GRAS) (Tajkarimi et al, 2010; Solorzano-Santos and Miranda-Novales, 2012). The mechanical, water solubility, water swelling, water vapor permeability, color properties of gelatin/ZMO films and antioxidant and antibacterial activities of the gelatin/ZMO were examined

Objectives

Methods

Results