Abstract
Visual rhodopsin forms an excellent archetype for studying spatial and temporal protein dynamics in G-protein-coupled receptors (GPCR) of the human genome. We have proposed a volumetric expansion in rhodopsin following its photoactivation [1]; however, a time-resolved description is lacking. Here we show the first time-resolved X-ray scattering-based detection of such an expansion of rhodopsin leading to the signaling state. We conducted time-resolved small-angle and wide-angle scattering (TR-SAXS and TR-WAXS, respectively) of rhodopsin solubilized in CHAPS detergent at the BioCARS synchrotron (Advanced Photon Source).
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