Abstract
We report a novel class of glutathione S-transferase (GST) from the model cyanobacterium Synechocystis PCC 6803 (sll1545) which catalyzes the detoxification of the water pollutant dichloroacetate and also shows strong glutathione-dependent peroxidase activity representing the classical activities of zeta and theta/alpha class respectively. Interestingly, sll1545 has very low sequence and structural similarity with these classes. This is the first report of dichloroacetate degradation activity by any bacterial GST. Based on these results we classify sll1545 to a novel GST class, rho. The present data also indicate potential biotechnological and industrial applications of cyanobacterial GST in dichloroacetate-polluted areas.
Highlights
Dichloroacetate (DCA), a by-product of water chlorination and a metabolite of drug and industrial chemicals is known to cause nerve and liver damage [1,2,3]
Thorough kinetic examination has not been performed with glutathione S-transferase zeta (GSTZ), we studied the kinetic parameters of sll1545 in detail
The structural alignments show major deviation between sll1545 and alpha/theta classes. Based on these results we propose sll1545 to be of a novel glutathione S-transferase (GST) class and name it as rho class
Summary
Dichloroacetate (DCA), a by-product of water chlorination and a metabolite of drug and industrial chemicals is known to cause nerve and liver damage [1,2,3]. It is a xenobiotic of interest to both environmental toxicologists and clinicians [3,4,5]. DCA stimulates the activity of pyruvate dehydrogenase enzyme complex of mitochondria, resulting in increased oxidation of blood glucose and lactate and can help in treatment of lactic acidosis [7,8,9,10]. A low dose of DCA is highly effective for treatment of congenital lactic acidosis [7]. Recent researches suggest that it may enhance cellular energy metabolism and can help in cancer treatment [11,12].
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