Functional chemical modification of a porcine acellular dermal matrix with a modified naturally derived polysaccharide crosslinker

Abstract

ABSTRACTAntibacterial and physicochemical properties are generally considered important features of the porcine acellular dermal matrix (pADM). Oxidized 2‐hydroxypropyltrimethyl ammonium chloride chitosan (OHTCC) was applied to crosslink with pADM at dosages of 1, 2, 4, 8, and 16%. The properties of the crosslinked pADM (OHTCC‐pADM) were evaluated. DSC and TG analysis suggested that crosslinking could promote the thermal stability, the highest Td, and Tmax of OHTCC‐pADM (8%) was 80 °C and 325 °C, which has been improved by 15 °C and 13 °C, respectively. While FTIR and AFM tests indicated that the structure integrity of collagen could still be maintained. SEM tests demonstrated the sustained three‐dimensional architecture of OHTCC‐pADM with appropriate porosity. Moreover, OHTCC‐pADM exhibited improved ability to resist collagenase degradation, the degradation rate of 4%‐, 8%‐OHTCC‐pADM was <50%. The tensile strength of OHTCC‐pADM proved to be superior compared to pADM. Furthermore, 8%‐OHTCC‐pADM exhibited nearly 90% antibacterial activity against Escherichia coli, and Staphylococcus aureus. In addition, the results of the 3‐(4,5‐dimethylthiazol‐2yl)‐2,5‐diphenyltetrazoliumbromide study showed that the cytocompatibility of OHTCC‐pADM decreased with the increasing the amount of OHTCC, but all relative proliferation rates were above 80%. In conclusion, our study revealed that OHTCC stabilized and functionalized pADM while preserving good cytocompatibility. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47633.