Porcine Acellular Dermal Matrix Modified by N-(2-Hydroxy) Propyl-3-Trimethyl Ammonium Chitosan Chloride

Abstract

Porcine acellular dermal matrix (pADM) was modified by N-(2-Hydroxy) propyl-3-Trimethyl Ammonium Chitosan Chloride (HTCC) with varied concentration, pH, time and temperature. The absorption of HTCC, surface state, hydrophilicity, antibacterial properties and cytotoxicity of pADM were researched before and after modification. Results show that the optimum experimental condition of modification is pH 6.4, 37°C, treated for 48 hours, and the concentration of HTCC 0.7%. The contact angle of modified pADM reduces and the water absorption ratio increases, but the tensile strength and moisture permeability falls. Obvious antibacterial action is observed on Escherichia Coli and Staphylococcus Aureus, and cytotoxicity is grade 2. This work suggests the feasibility of HTCC to modify pADM.