Functional characterization of HSFs from wheat in response to heat and other abiotic stress conditions.

Abstract

High temperature stress is known to be one of the major limiting factors for wheat productivity worldwide. HSFs are known to play a central role in heat stress response in plants. Hence, the current study is an attempt to explore an in-depth involvement of TaHSFs in stress responses mainly in heat and other abiotic responses like salinity, drought, and cold stress. Effort was made to understand as how the expression of HSF is able to define the differential robustness of wheat varieties. Subsequent studies were done to establish the involvement of any temporal or spatial cue on the behavior of these TaHSFs under heat stress conditions. A total of 53 HSFs have been reported until date and out of these, few TaHSFs including one identified in our library, i.e., TaHsfA2d (Traes_4AS_52EB860E7.2), were selected for the expression analysis studies. The expressions of these HSFs were found to differ in both magnitude and sensitivity to the heat as well as other abiotic stresses. Moreover, these TaHSFs displayed wide range of expression in different tissues like anther, ovary, lemma, palea, awn, glume, and different stages of seed development. Thus, TaHSFs appear to be under dynamic expression as they respond in a unique manner to spatial, temporal, and environmental cues. Therefore, these HSFs can be used as candidate genes for understanding the molecular mechanism under heat stress and can be utilized for improving crop yield by enhancing the tolerance and survival of the crop plants under adverse environment conditions.