Abstract
Using functional polysaccharides as elicitors to induce plant disease resistance has emerged as an effective plant protection strategy. In this study, we found that crude polysaccharides derived from by-products of Ganoderma lucidum can effectively prevent and control the Tomato Yellow Leaf Curl Virus (TYLCV). As a result, we isolated and purified a homogeneous polysaccharide known as glucan-like peptide (GLP1), from crude polysaccharide of G. lucidum and explored the mechanism behind GLP1's ability to induce resistance in tomatoes against TYLCV. Analysis of the monosaccharide composition and high-performance gel permeation chromatography (HPGPC) revealed that GLP1 is solely composed of glucose, with molecular weight (Mw), 7.367 KDa, a number-average molecular weight (Mn) of 6.876 KDa, and peak molecular weight (Mp) values 7.505 KDa. Fourier transform infrared spectroscopy indicated that GLP1 may have a pyranose structure with an α-glycosidic bond. Plant hormones play a crucial role in disease resistance, and we found that GLP1 affects hormone levels in tomato plants by increasing jasmonic acid (JA) and ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Additionally, GLP1promoting the expression of genes associated with the ethylene signaling pathway. When inhibitors of ethylene synthesis blocked ethylene signaling, the resistance of tomato to TYLCV was significantly reduced. These findings suggest that ethylene signaling is involved in GLP1-induced tomato resistance and likely interacts with other pathways to contribute to this resistance.
Published Version
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