Abstract
Drought stress can trigger the production of ABA in plants, in response to adverse conditions, which induces the transcript of stress-related marker genes. The R2R3 MYB TFs are implicated in regulation of various plants developmental, metabolic and multiple environmental stress responses. Here, a R2R3-MYB cloned gene, GaMYB62L, was transformed in Arabidopsis and was functionally characterized. The GaMYB62L protein contains two SANT domains with a conserved R2R3 imperfect repeats. The GaMYB62L cDNA is 1,017 bp with a CDS of 879, encodes a 292-residue polypeptide with MW of 38.78 kD and a pI value of 8.91. Overexpressed GaMYB62L transgenic Arabidopsis have increased proline and chlorophyll content, superior seed germination rate under salt and osmotic stress, less water loss rate with reduced stomatal apertures, high drought avoidance as compared to WT on water deprivation and also significant plant survival rates at low temperature. In addition, overexpressed GaMYB62L lines were more sensitive to ABA mediated germination and root elongation assay. Moreover, ABA induced GaMYB62L overexpression, enhanced the expression of ABA stress related marker genes like RD22, COR15A, ADH1, and RD29A. Together, overexpression of GaMYB62L suggested having developed better drought, salt and cold tolerance in transgenic Arabidopsis and thus presented it as a prospective candidate gene to achieve better abiotic stress tolerance in cotton crop.
Highlights
Environmental abiotic factors especially drought stress severely effects the global production and distribution of important cash crops [1, 2]
Our laboratory previous studies on RNA-seq data of Gossypium arboreum revealed to have hormone crosstalk, when MYB TF are implicated in modifying plant response toward drought and NaCl stresses in different tissues [33]
The alignment results between cDNA and genomic sequence retrieved from cotton genome project database available online demonstrated that GaMYB62L contains 3 introns (S1C Fig) and is located on chromosome 6 with cotton ID number cotton_A_01409
Summary
Environmental abiotic factors especially drought stress severely effects the global production and distribution of important cash crops [1, 2]. To overcome these adverse conditions plants have evolutionarily developed a signaling cascade of relaying stress sensors, signaling transduction pathways, TFs and downstream targets, with an output of regulatory gene products and metabolites. Numerous stress responsive marker genes and promoter genes related to abiotic and biotic stress have been broadly researched in important agronomic plants [5, 6]. Based on DNA-binding domains (DBD) TFs are divided into MYB/MYC TFs, DREB TFs, WRKY TFs, NAC TFs, AP2/ERF TFs and bZIP TFs [7, 8]
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