Functional changes in human peripheral neutrophils in workers with different exposure to noxious agents

Abstract

Peripheral neutrophils (PMN) are a useful model to study cell activation and to evaluate the multiple stressors' interference with signalling pathways. This study aimed to evaluate the effects of different exposure to noxious agents on PMN activity by chemiluminescence assays. Two groups of office workers were recruited: peripheral whole blood cells and isolated PMNs were analyzed, performing intra- and extra-cellular chemiluminescence evaluation to study compartmental release of radical species. The exposed group shows a higher emission in resting whole blood chemiluminescence than in controls; the intracellular chemiluminescence emission in urban employees was increased, whereas the extracellular was not affected. After stimulation, the extracellular chemiluminescence was decreased in urban employees, whereas the intracellular emission did not show differences. We hypothesize that once stimulated, exposed subjects' PMNs respond to stimuli less than unexposed: PMNs could be considered indicators of chronic exposure to noxious agents.