Abstract
Mutants of Coprinus lagopus unable to utilise acetate were selected on a glucose medium containing the toxic analogue fluoroacetic acid. The mutants map at three unlinked loci, designated acu-1, acu-11, and acu-12. Mutations at the acu-1 locus lead to loss of acetyl-CoA synthetase activity; the functions of the other two loci were not resolved but were shown not to involve either this enzyme or isocitrate lyase. Mutants at different loci showed normal complementation when combined in pairs in dikaryons or heterozygous diploids showing that there is no localised intranuclear activity of the gene products involved. Tests for interallelic complementation between twenty-five differently isolated acu-1 mutants proved negative, indicating that the acetyl-CoA synthetase activity is carried on a single polypeptide.
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