Function of Gene 49 of Bacteriophage T4 III, Isolation of Holliday Structures from Very Fast-Sedimenting DNA

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Branched DNA molecules were identified in ClaI digests of cytosine containing very fast-sedimenting DNA (VFSDNA CYT) which was isolated from Escherichia coli infected with the multiple mutant 49 -GT7 of phage T4. In about 10% of randomly picked ClaI fragments branches with three arms (Y-structures) as well as four arms (Holliday structures) were seen in the electron microscope. Branched structures were absent from ClaI digests after treatment with purified endonuclease VII (gp49) in vitro.