Abstract
Background: Lung adenocarcinoma (LUAD), the most prevalent type of lung cancer, is often metastatic and has a poor prognosis. Recent studies have demonstrated an important role for fucosyltransferase 8 (FUT8) in carcinogenesis and cancer progression. Methods: A meta-analysis with 15 eligible datasets from Gene Expression Omnibus (GEO) was performed to explore the expression of FUT8 in LUAD. The results were further verified in The Cancer Genome Atlas (TCGA) database, followed by survival analysis using Kaplan-Meier plotter. We also validated the protein expression of FUT8 by immunohistochemistry (IHC). In vitro experiments were conducted to determine the biological effects of FUT8 in LUAD cells. Results: The meta-analysis showed the FUT8 expression in LUAD tissues was significantly higher than those in normal lung tissues [standard mean difference (SMD): 1.40; 95% confidence interval (CI): .95–1.85]. The results of TCGA database verified the expression of FUT8 increased in LUAD tissues versus normal tissues. IHC analyses indicated that the protein levels of FUT8 were up-regulated in LUAD, and elevated FUT8 expression was significantly correlated with poor prognosis in LUAD patients. Multivariable Cox regression analysis revealed that FUT8 expression was an independent prognostic factor. Besides, in vitro experiments showed that knockdown of FUT8 in LUAD cells markedly restrained cell proliferation, and stimulated cell apoptosis. Conclusion: This study indicates that increased FUT8 expression is correlated with shortened survival of LUAD patients and might favor the progression of the disease.
Highlights
In China, lung cancer is the most common cause of cancer-related deaths in both men and women [1]
IHC analyses indicated that the protein levels of fucosyltransferase 8 (FUT8) were up-regulated in Lung adenocarcinoma (LUAD), and elevated FUT8 expression was significantly correlated with poor prognosis in LUAD patients
This study indicates that increased FUT8 expression is correlated with shortened survival of LUAD patients and might favor the progression of the disease
Summary
In China, lung cancer is the most common cause of cancer-related deaths in both men and women [1]. It is estimated that 774,323 new lung cancer cases occurred in China in 2018, ranking first among all cancer types. Lung adenocarcinoma (LUAD) is the most frequently diagnosed histologic subtype of lung cancer, representing 38.5% of all pulmonary neoplasm cases. Most patients with non-small cell lung cancer (NSCLC) are diagnosed at advanced stages and have a dismal 5-year survival rate of 6.9% [3]. The identification of common driver mutations, such as epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase, leads to the development of many novel targeted therapies that has profoundly changed the survival outcomes of NSCLC patients, especially those with LUAD [4]. Lung adenocarcinoma (LUAD), the most prevalent type of lung cancer, is often metastatic and has a poor prognosis. Recent studies have demonstrated an important role for fucosyltransferase 8 (FUT8) in carcinogenesis and cancer progression
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