FİTOYIĞINTIDAN EKSTRAKTIN OPTİMAL ALINMA TEXNOLOGİYASININ SEÇİLMƏSİ VƏ ONUN BƏZİ GÖSTƏRİCİLƏRİNİN TƏYİNİ

Abstract

The article is devoted to the choice of the optimal technology for obtaining an extract prepared on the basis of a plant complex, which includes dog rose hips (Rosa canina L.), calendula officinalis flowers (Calendula officinalis), rosemary officinalis (Rosmarinus officinalis L.), and the determination of some of its indicators. To obtain an extract from phytomass, extraction was carried out by maceration and percolation using ethanol as an extractant (40%, 70%, and 95%). In the extracts obtained, the identity of rutoside was determined and its quantitative determination was carried out by the spectrophotometric method. According to the results of quantitative determination, the amount of flavonoids in the extract obtained by the maceration method was higher, and the relative error of the determination was  1.12%, and the relative error of the extract obtained by the percolation method was  3.47. %. As a result, the maceration method was chosen as the extraction method. In order to study the initial antibacterial and antifungal activity of the extract obtained by the maceration method, comparative microbiological studies were carried out using the disk diffusion method. It has been established that the presented extract has a zone of inhibition against S. aureus and Candida albicans cells. Thus, taking into account the results of microbiological studies, 70% ethanol was chosen as the optimal solvent.