FTIR SPECTRUM AND XRD OF POSTBIOTICS-EXOPOLYSACCHARIDES ZINC OXIDE NANOPARTICLES

Abstract

The human body is occupied by millions of living microorganisms which all together, are called the human micro biota. Exopolysaccharides derived from lactic acid bacteria play a crucial role in improving the rheology, texture, mouth feel of fermented food formulations in food industry. The objective of the study was to characterise zinc oxide nanoparticles from postbiotics-exopolysaccharides and analyse the FTIR Spectrum and XRD of Postbiotics–Exopolysaccharides Zinc Oxide Nanoparticles. Postbiotics-exopolysaccharides was isolated from probiotic Lactobacillus culture medium using centrifugation method. Postbiotics-exopolysaccharides zinc oxide nanoparticles was synthesised by direct precipitation method using zinc sulphate and sodium hydroxide as precursor and characterisation was done using Fourier Transformed Infrared Spectroscopy (FTIR) and X-Ray Diffraction analysis. Results revealed that 46% of carbohydrate and 10% of protein content confirmed the presence of exopolysaccharides and in particular postbiotics-exopolysaccharides. The peak at 346.3 nm by Ultraviolet-Visible spectroscopy confirmed the presence of nanoparticles. The functional groups of postbiotics-exopolysaccharides zinc nanoparticles was recorded by Fourier Transform Infrared Spectroscopy using SHIMADZU Miracle spectrophotometer (FTIR 820IPC) KBr techniques. The scanning electron micrograph confirmed that postbiotics-exopolysaccharides zinc oxide nanoparticles had a rough, rigid and compact surface structure. The sharp narrow peak obtained in the graph by X-ray Diffraction Analysis confirmed the crystalline structure. The crystalline size of the postbiotics-exopolysaccharides zinc oxide nanoparticles as calculated by Debye-Scherer equation D=K λ/ β (Cos θ) with highest peak value 34.01 confirmed the crystalline size to be 33.31 nm.