Fructose Intake Impairs the Synergistic Vasomotor Manifestation of Nitric Oxide and Hydrogen Sulfide in Rat Aorta.

Andrea Berenyiova,Samuel Golas,Magdalena Drobna,Sona Cacanyiova,Martina Cebova

doi:10.3390/ijms22094749

Abstract

In this study, we evaluated the effect of eight weeks of administration of 10% fructose solution to adult Wistar Kyoto (WKY) rats on systolic blood pressure (SBP), plasma and biometric parameters, vasoactive properties of the thoracic aorta (TA), NO synthase (NOS) activity, and the expression of enzymes producing NO and H2S. Eight weeks of fructose administration did not affect SBP, glycaemia, or the plasma levels of total cholesterol or low-density and high-density lipoprotein; however, it significantly increased the plasma levels of γ-glutamyl transferase and alanine transaminase. Chronic fructose intake deteriorated endothelium-dependent vasorelaxation (EDVR) and increased the sensitivity of adrenergic receptors to noradrenaline. Acute NOS inhibition evoked a reduction in EDVR that was similar between groups; however, it increased adrenergic contraction more in fructose-fed rats. CSE inhibition decreased EDVR in WKY but not in fructose-fed rats. The application of a H2S scavenger evoked a reduction in the EDVR in WKY rats and normalized the sensitivity of adrenergic receptors in rats treated with fructose. Fructose intake did not change NOS activity but reduced the expression of eNOS and CBS in the TA and CSE and CBS in the left ventricle. Based on our results, we could assume that the impaired vascular function induced by increased fructose intake was probably not directly associated with a decreased production of NO, but rather with impairment of the NO–H2S interaction and its manifestation in vasoactive responses.

Highlights

Accepted: 27 April 2021Currently, significant changes in nutritional habits present an important factor encroaching into the equilibrium of different signaling pathways in the body
The eight-week fructose treatment did of heart weighttreatment to body weight nor tibial length to body weight were changed after fructose treatment the eight-week did not affect affect the weight(Table gain 1)
We revealed a significant effect of the eight-week fructose administration on the values of systolic blood pressure, there were no differences between the experimental groups in individual weeks (Figure 1)

Summary

Introduction

Accepted: 27 April 2021Currently, significant changes in nutritional habits present an important factor encroaching into the equilibrium of different signaling pathways in the body. Excessive intake of added sugars, mainly as sugar-sweetened beverages, has been implicated in the development of obesity and metabolic disturbances, increasing the risk of cardiovascular disease mortality. Two major sweeteners are predominantly used in the food and beverage industry: sucrose, a disaccharide containing 50% fructose and 50% glucose; and high-fructose corn syrup, which mainly consists of 55% fructose and 45% glucose [1,2]. Due to its specific metabolism, predominantly in the liver, fructose is more lipogenic than glucose. Long-term application of this saccharide at different concentrations in food or drinking water has been used to induce metabolic disorders in experimental models [3]. Animals chronically receiving high amounts of fructose developed hypertriglyceridemia, insulin resistance, hyperinsulinemia, and often obesity and hypertension [4,5,6]

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