Abstract
Summary Callus derived from poplar (Populus nigra L.) or from winter rape (Brassica napus L. var. oleifera) plants was exposed to freezing temperature in a range of -2°C to -12°C. The frost-induced injuries were estimated either by an electro-conductivity method, by the TTC reduction test or by determination of the ability of the tissue to regrow on a fresh medium after freezing. Comparison of the results obtained indicates that poplar callus has a higher frost resistance than rape callus. In both kinds of tissue, membrane permeability alterations higher than 20% (as detected with the conductivity method) seem to be deleterious for the further functioning and regrowth abilities of callus.
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