Abstract
Most quantitative analysis in chromatography is performed based on the peak area, the integrated area underneath the curve defining a peak. There are many variables and settings in a data system that can impact this determination. In this installment, we will examine several of the common parameters that can affect automated peak integration and the resulting peak areas. We will consider how the data system detects the beginning and end of the peak, how it determines the peak maximum, how real peaks are differentiated from noise, and how signals at individual time intervals are summed to generate the peak area. We will also briefly look at techniques for determining the areas of unusually shaped peaks.
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