Frequency of Mia (MNS7) and Classification of Mia-Positive Hybrid Glycophorins in an Australian Blood Donor Population

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Background: MNS blood group system genes GYPA and GYPB share a high degree of sequence homology and gene structure. Homologous exchanges between GYPA and GYPB form hybrid genes encoding hybrid glycophorins GP(A-B-A) and GP(B-A-B). Over 20 hybrid glycophorins have been characterised. Each has a distinct phenotype defined by the profile of antigens expressed including Mi^a. Seven hybrid glycophorins carry Mi^a and have been reported in Caucasian and Asian population groups. In Australia, the population is diverse; however, the prevalence of hybrid glycophorins in the population has never been determined. The aims of this study were to determine the frequency of Mi^a and to classify Mi^a-positive hybrid glycophorins in an Australian blood donor population. Method: Blood samples from 5,098 Australian blood donors were randomly selected and screened for Mi^a using anti-Mi^a monoclonal antibody (CBC-172) by standard haemagglutination technique. Mi^a-positive red blood cells (RBCs) were further characterised using a panel of phenotyping reagents. Genotyping by high-resolution melting analysis and DNA sequencing were used to confirm serology. Result: RBCs from 11/5,098 samples were Mi^a-positive, representing a frequency of 0.22%. Serological and molecular typing identified four types of Mi^a-positive hybrid glycophorins: GP.Hut (n = 2), GP.Vw (n = 3), GP.Mur (n = 5), and 1 GP.Bun (n = 1). GP.Mur was the most common. Conclusion: This is the first comprehensive study on the frequency of Mi^a and types of hybrid glycophorins present in an Australian blood donor population. The demographics of Australia are diverse and ever-changing. Knowing the blood group profile in a population is essential to manage transfusion needs.