Abstract
A species of blue-green alga, Spirulina platensis, is extremely susceptible to freezing and drying. However, young cells grown autotrophically with a high intensity of light were resistant to freeze-thawing if the rate of temperature change in the operation was in the range of 20–50 °C/min. Several amino acids, gum arabic, and gelatin were effective in protecting cells from injury caused by freezing. In the case of drying only gum arabic and gelatin could protect cells from injury. The gum arabic-plug method of freeze-drying was shown to be the most suitable method for the maintenance of viability in this alga. The freeze-thawed or freeze-dried cells grew to form abnormally long cells after a period of long lag in the first stage of transfer.
Published Version
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