Freeze-Dried human platelet-rich plasma effect on osteoblast number and collagen density in periodontitis treatment

Abstract

Objective: This study aimed to determine the effect of FD-hPRP on the number of osteoblast and collagen density in periodontitis treatment by a histomorphometric study on oryctolagus cuniculus rabbits' alveolar bone and periodontal ligament. Material and Methods: Sixteen male rabbits were randomly divided into four groups: Normal; Periodontitis; FD-hPRP; DFDBA. After periodontal surgery by open flap debridement, FD-hPRP was applied in group 3, and DFDBA was applied in group 4. Then all rabbits were decapitated eight weeks after surgery. Histomorphometric analysis calculates the number of osteoblasts and collagen density using image J software. Data were analyzed using one-way ANOVA followed by Post Hoc Least Significant Difference (LSD) test. Results: The results showed a significant difference (p>0.05) between the periodontitis and FD-hPRP and also DFDBA group. Conclusion: The application of FD-hPRP effect increases the number of osteoblast and collagen density in periodontitis treatment histomorphometrically in rabbit’s alveolar bone and periodontal ligament.