Free Radical Scavenging Capacities and Alleviating Actions of Polysaccharides Extract of Termitomyces le-testui on Methylprednisolone-Induced Immunodepression in Rats.

Foncham Evans Ngwenah,Tume Christopher,Kada Sanda Antoine,Salah Martin,Oumar Mahamat

doi:10.1155/2021/5893210

Abstract

Background Natural products have been said to show immunomodulatory and antioxidant activities. The research study was aimed to assess the immunomodulatory and free radical scavenging activities of crude polysaccharide from dry mushroom fruiting bodies of Termitomyces le-testui. Materials and Methods Hot water extract of polysaccharide extract of T. le-testui was prepared and tested in white albino Wister rats for its immunomodulatory activities effect on methylprednisolone-immunosuppressed animals. In addition, the radical scavenging activity of the polysaccharide was evaluated using nitrite and hydrogen peroxide. Results The result of the study showed that the polysaccharide T. le-testui increases the phagocytic index, energy metabolism of macrophages, spleen index, and nitric oxide in a concentration-dependent manner in immunosuppressed animals. Also, it was observed that the extract increased dose-dependent total oxidative stress and thymus index. Finally, the crude polysaccharide-rich extract showed nitrite and hydrogen peroxide scavenging activity in a concentration-dependent manner. Conclusion Polysaccharide-rich extract possesses immunomodulatory and antioxidant properties.

Highlights

Natural products, especially plants, are one of the immunomodulators that can provide an alternative to conventional chemotherapy for a large variety of diseases
Nitrite and Hydrogen Peroxide Scavenging Activity of Crude Polysaccharides of T. le-testui. e polysacchariderich extract of T le-testui was tested for its antiradical activity by evaluating its effect on nitrite and hydrogen peroxide scavenging activity (Table 1). e result showed that a concentration-dependent increase of the inhibition percentage of free radical activity was observed with extract
Free Radicals Scavenging Using iobarbituric Acid (TBA) Method. e thiobarbituric acid (TBA) method was used to evaluate the inhibition of the production of carbonyl compounds degraded from the peroxides at a later stage. e results showed that the polysaccharides active extracts of T. le-testui caused a decrease in the production of carbonyl compounds in a concentration-dependent manner (Figure 2). e production of carbonyl compounds was significantly less with the polysaccharides extract than the synthetic antioxidant vitamin C

Summary

Introduction

Especially plants, are one of the immunomodulators that can provide an alternative to conventional chemotherapy for a large variety of diseases. As immunomodulators, they are especially important when the host defense mechanism has to be stimulated in cases of the impaired immune response. Is is partly due to a large number of substances with potential immunomodulatory activities that mushrooms possess [2, 3]. E result of the study showed that the polysaccharide T. le-testui increases the phagocytic index, energy metabolism of macrophages, spleen index, and nitric oxide in a concentration-dependent manner in immunosuppressed animals. The crude polysaccharide-rich extract showed nitrite and hydrogen peroxide scavenging activity in a concentration-dependent manner.

Methods

Results

Conclusion