Free radical scavenging activity of Castanopsis indica in mediating hepatoprotective activity of carbon tetrachloride intoxicated rats

Abstract

ObjectiveTo investigate the free radical scavenging activity of methanol extract of Castanopsis indica (C. indica) in mediating hepatoprotective activity of carbon tetrachloride intoxicated rats. MethodsFree radical scavenging activity was evaluated by using DPPH, nitric oxide, peroxynitrite, superoxide radical, hydroxyl radical, hypochlorous acid scavenging and lipid peroxidation assay models. ResultsThe IC50 values of C. indica in DPPH, nitric oxide, peroxynitrite, superoxide radical, hydroxyl radical, hypochlorous acid scavenging and lipid peroxidation were found to be (43.86±1.85), (103.20±2.41), (327.00±7.23), (74.76±2.50), (35.39±1.51), (193.1m±6.74), (42.30±2.46) μg/mL, respectively. There was also a dose dependant increase in reductive ability of C. indica extract with increase in concentration. In case of hepatoprotective evaluation the levels of liver enzymatic, non-enzymatic systems (SGOT, SGPT, ALP), total bilirubin, total protein, catalase (CAT), reduce glutathione (GSH), superoxide dismutase and lipid peroxidation were restored towards the normal value in C. indica treated carbon tetrachloride intoxicated rats. The free radical scavenging and antioxidant activities may be attributed to the presence of phenolic compound, which was (318.70±1.39) μg in 1 000 μg of C. indica extract. ConclusionsResults obtained in the present study indicate that the C. indica is a potential hepatoprotective agent via its free radical scavenging.