Abstract
Free and monosulphated 5α-androst-16-en-3α-ol, 5α-androst-16-en-3β-ol and 5,16-androstadien-3β-ol were identified and quantified in human cadaver testis tissue. Free and monosulphated steroid fractions were isolated by Sephadex LH-20 chromatography. Further separation and purification of the 16-unsaturated C 19 steroids was achieved using silicic acid and hydroxyalkoxypropyl Sephadex chromatography. The steroids were finally identified by gas-liquid chromatography and gas chromatography-mass spectrometry. In the free steroid fraction the relative abundance of the 16-unsaturated C 19 steroids was 5α-androst-16-en-3β-ol > 5,16-androstadien-3β-ol > 5α-androst-16-en -3α-ol and in the monosulphate fraction 5,16-androstadien-3β-ol > 5α-androst-16-en-3β-ol > 5a-androst-16-en-3α-ol.
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