Förderung der DNS-synthese in pflanzlichen zellen durch vitamin a-säure

Abstract

This paper shows that for the first time in plant material retinoic acid (vitamin A acid, VAS) significantly enhanced in vivo incorporation of [6- 3 H]-as well as [ methyl- 3 H] thymidine into DNA in plant cells. In carrot cell suspension cultures VAS concentrations from 10 −10 to 10 −5 M dissolved in DMSO to facilitate the uptake of this compound were applied to the cells 30 min prior to the addition of thymidine. VAS and DMSO remained in the system also during the 6 h incubation with thymidine. The incorporation of the labeled nucleoside was enhanced up to 170% at a VAS concentration of 10 −6 M. In cultured Cymbidium protocorms the short pretreatment alone rapidly increased DNA synthesis, but prolonged presence had no such effect. The culture of carrot cells in the presence of VAS throughout the 14-day culture period revealed a strong inhibition of growth at a 10 −15 M concentration and death of the cells at higher concentrations.