Abstract
Plant transformation systems based on gene vectors derived from the Ti plasmid of Agrobacterium tumefaciens (16), on direct DNA uptake (6), and on wheat dwarf virus (WDV) DNA (14) were used to introduce a number of chimeric genes into, respectively, tobacco protoplasts, suspension culture cells of Triticum monococcum, and Zea mays. The actual genes involved in these studies were chosen to serve as models for investigating the fate, expression, recombination, and transposition of foreign DNA in plant cells.
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