Abstract
We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. OPT relies on precise mechanical rotation and is less mechanically stable than large-scale computer tomography (CT) scanning systems, leading to reconstruction artefacts. The algorithm uses multiple (5+) tracked fiducial beads to recover the sample pose and the image rays are then back-projected at each orientation. The quality of the image reconstruction using the proposed algorithm shows an improvement when compared to the Radon transform. Moreover, when adding a systematic spatial and angular mechanical drift, the reconstruction shows a significant improvement over the Radon transform.
Highlights
We present a tomographic reconstruction algorithm, which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift
For the volume reconstruction procedure, there is a fitting step to recover the path of the fiducial marker, which is used to correct the sinogram before applying the inverse Radon transform
The quality of the reconstructions shows a slight improvement when compared to the standard Radon transform, with a great effect when a systematic drift is introduced
Summary
We present a tomographic reconstruction algorithm (flOPT), which is applied to Optical Projection Tomography (OPT) images, that is robust to mechanical jitter and systematic angular and spatial drift. For the volume reconstruction procedure, there is a fitting step to recover the path of the fiducial marker, which is used to correct the sinogram before applying the inverse Radon transform.
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