Abstract
Optical projection tomography (OPT) is the direct optical equivalent of X-ray computed tomography (CT). To obtain a larger depth of field, traditional OPT usually decreases the numerical aperture (NA) of the objective lens to decrease the resolution of the image. So, there is a trade-off between sample size and resolution. Commercial microfluidic systems can observe a sample in flow mode. In this paper, an OPT instrument is constructed to observe samples. The OPT instrument is combined with commercial microfluidic systems to obtain a three-dimensional and time (3D + T)/four-dimensional (4D) video of the sample. “Focal plane scanning” is also used to increase the images’ depth of field. A series of two-dimensional (2D) images in different focal planes was observed and compared with images simulated using our program. Our work dynamically monitors 3D OPT images. Commercial microfluidic systems simulate blood flow, which has potential application in blood monitoring and intelligent drug delivery platforms. We design an OPT adaptor to perform OPT on a commercial wide-field inverted microscope (Olympusix81). Images in different focal planes are observed and analyzed. Using a commercial microfluidic system, a video is also acquired to record motion pictures of samples at different flow rates. To our knowledge, this is the first time an OPT setup has been combined with a microfluidic system.
Highlights
Three-dimensional imaging has become an effective tool for biomedical research
This gap was filled by optical projection tomography (OPT) technology
OPT is capable of utilizing many colored and fluorescent dyes that were developed for tissue-specific or gene-specific staining, which is important to three-dimensional observations of specific tissues because it allows a computer to automatically determine the three-dimensional structure of the target tissue
Summary
Three-dimensional imaging has become an effective tool for biomedical research. A gap between macroscopic imaging technology and microscopic imaging technology led to an inability to observe samples of certain sizes. This gap was filled by optical projection tomography (OPT) technology. OPT technology enables three-dimensional imaging of samples that are 1–10 mm in size. Samples of this size are too large for confocal imaging, and too small for magnetic resonance imaging (MRI), but most vertebrate embryos are in this size range [1,2]. OPT microscopy is especially suitable for imaging samples whose size lies between 0.5 mm and 10 mm. Many universities [3,4,5,6,7,8,9,10,11] have adopted OPT systems to help them do biomedical research
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