Abstract
A sequential scheme for the fractionation of yeast transfer RNAs by chromatography on the polysaccharide gel media Sepharose 4B and Ultrogel AcA 44 in reversed concentration gradients of ammonium sulphate is described and the optimum conditions worked out. Four species, tRNA Met, tRNA Asn, tRNA Pro and tRNA Ala are obtained in a highly purified form, while several other acceptor species are highly concentrated in the subfractions. The mechanism of this chromatographic system is discussed.
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