Abstract
IN the step-wise analysis of nucleotide sequence in tobacco mosaic virus ribonucleic acid (TMV–RNA)1, occasional random splitting of internucleotide bonds results in the production of partially degraded molecules. These broken chains give rise to additional and, of course, false end-groups. In order that the sequence analysis be meaningful it is necessary that a method for the removal of broken chains be developed. We have looked into the possibility of separating undegraded from partially degraded TMV–RNA molecules by chromatography on methylated serum albumin–kieselguhr (MAK) columns2. Such a fractionation may also be of use in the reverse situation where it is desired to eliminate any intact molecules from a deliberately inactivated RNA preparation.
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