Abstract
Native and reconstituted chromatin from Ehrlich ascites tumor cells were fractionated into template active and inactive fractions by the DNase II/Mg++ - solubility method of Gottesfeld and colleagues (1974). The Mg++-soluble (template active) fractions were compared in respect to sedimentation behaviour in sucrose gradients and the relative content of specific transcribed (ribosomal) and non-transcribed (satellite) DNA sequences. It was found that the Mg++-soluble fraction of the native chromatin was enriched in ribosomal DNA while almost completely devoid of satellite DNA; the nucleoprotein monomer of this fraction sedimented in sucrose gradient at 14s. Similar results were obtained if chromatin was fractionated in the presence of 3 M urea. With reconstituted chromatin, however, neither the sedimentation profile, nor the relative content of ribosomal and satellite DNA sequences were recovered, thus indicating that reconstitution did not yield nucleoprotein structurally similar to native chromatin.
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