Abstract

In this Letter, a field programmable gate array (FPGA)-based synchronising control system design for spinning disk confocal image scanning microscope is described. Based on the analysis on the condition of laser triggering, the author simplifies the design by transforming the floating-point calculation formula to a very simple form of integer calculation, which can then be implemented by fixed-point calculation on FPGA without trimming any bit, for which there is no computational precision loss. The testing of simulation and experiment shows that the design is very accurate and reliable.

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