Formulation of Traditional Mask Powder Containing the Mixture of Coffea robusta, Angelica keiskei and Oryzae sativa, and its Activity as Tyrosinase Enzyme Inhibitor

Diah Lia Aulifa,Nur Asni Setiani,Arif Budiman,Annisa Sri Rahayu Berliana

doi:10.5530/pj.2021.13.205

Abstract

Formulation of Traditional Mask Powder Containing the Mixture of Coffea robusta, Angelica keiskei and Oryzae sativa, and its Activity as Tyrosinase Enzyme Inhibitor

Highlights

Skin aging and loss of tensile strength can cause wrinkles, roughness, and dryness.[1,2] hyperpigmentation occurs due to the overproduction of melanin[3], and the accumulation and overproduction of melanin can lead to a serious aesthetic problem due to age spots or melasma.[4,5] The enzyme tyrosinase is the key factor in the synthesis of melanin.[3]
A total of 0.5 mL of each sample was dissolved in methanol (CR bean was 1000 ppm, Angelica keiskei (AK) leaf was 700 ppm and Oryzae sativa (OS) was 4000 ppm) and mixed with 5 mL Folin-Ciocalteu reagent diluted with distilled water (1:10) and 4 mL Na2CO3 (1 M)
The specify limits on total yeast and mold counts was 1 x 104 CFU/gram, while for bacteria was 1 x 106 CFU/gram.[21]. These findings indicated that traditional mask powder from the mixture of Coffea robusta L. (CR) bean, AK leaf and OS was safe to be used as a topical preparation, especially as a skin lightening agent

Summary

INTRODUCTION

Skin aging and loss of tensile strength can cause wrinkles, roughness, and dryness.[1,2] hyperpigmentation occurs due to the overproduction of melanin[3], and the accumulation and overproduction of melanin can lead to a serious aesthetic problem due to age spots or melasma.[4,5] The enzyme tyrosinase is the key factor in the synthesis of melanin.[3]. Angelica keiskei (AK) recognized as a medicinally important herb in Asia is a plant that has a potential source as an antioxidant and tyrosinase enzyme inhibitor.[6,10] Chalcones, which has two aromatic rings with an unsaturated chain, is one of the popular groups of compounds in AK that can prevent overproduction of melanin by tyrosinase enzyme inhibition.[11] The previous studies reported that the isoprenoid-substituted moiety content in AK leaf extract increased the flavonoid's ability to inhibit melanin biosynthesis.[12] The xanthoangelol and 4-hydroxyderricin from AK extract can inhibit tyrosinase enzyme with IC50 values of 15.87 ± 1.21 μM and 60.14 ± 2.29 μM, respectively.[13]. The effect of CR bean, AK leaf and OS, alone, on tyrosinase enzyme activity was examined, and that the determined phenolic content was assumed to have a potential as tyrosinase enzyme inhibitory agent

MATERIALS AND METHODS

AND DISCUSSION

CONCLUSION