Formulation and Evaluation of Ethosomal Cream containing Resveratrol for the Treatment of Contact Dermatitis

Abstract

Objective: The aim of this study was to formulate and evaluate ethosomal cream containing resveratrol for the treatment of contact dermatitis. Method: Resveratrol-loaded ethosome was prepared using cold method. The prepared ethosomes were assessed for entrapment efficiency (%), TEM analysis and sedimentation rate to determine the optimal formulation for loading into cream base. The selected formulation was further investigated for particle size distribution and zeta potential. A cream base was formulated using fusion and trituration method and selected for loading with the ethosomal suspension, meeting specification suitable for topical delivery. Ethosomal cream formulations were evaluated for drug content, ex -vitro diffusion by using goat skin, skin retention, microbiological study and stability study. Result and Discussion: The formulation E3 was selected for loading in the optimized cream base (F4) to obtain a topical cream containing ethosomes of resveratrol. The release study reveals that release was controlled for the 24 hours. The drug release in buffer was less than 17% from all the formulations after 24 hours. The drug content of resveratrol in goat skin and cream remained in donor compartment indicates that the drug retention in skin was high in all the formulations, which is desirable for the topical formulations. The best possible release kinetics of the formulation loaded with resveratrol containing ethosomes was found to be zero order. The ‘n’ value from Peppas’s model indicated that the drug release was by Fickian diffusion. Stability study indicates that all the cream formulations were stable after a time period of one month. Conclusion: The present study conclusively supported that ethosomal cream containing resveratrol to be an advantageous topical drug delivery system in treatment of ACD. However detail in- vivo study should be conducted in future to be justify the in- vitro study. Keywords: Resveratrol, Ethosome, Cream, Contact dermatitis.