Formulation and Evaluation of Anti-Acne Cream Using the Extracts of Oscillatoria sp: Investigating its Bio compatibility and Toxicity by Skin irritation test and Brine Shrimp Lethality assay

Abstract

Acne by definition is a multifactorial continual inflammatory ailment of pilosebaceous units. Although antibiotics play an essential role in acne control, the development of drug resistance among acne-causing bacteria (Propionibacterium acne and Streptococcus sp)had created a need for the development of novel antimicrobial agents. Therefore, the present study focuses on the development of anti-acne cream using extracts of Oscillatoria sp. Oscillatoria extracts were used as a major constituent for acne cream with paraffin wax as the cream base. The Oscillatoria extracts and developed cream were investigated for antibacterial activity against acne-causing bacteria. The biocompatibility of the acne cream was evaluated by a patch test and Brine shrimp lethality assay was performed to determine the toxicity of the developed cream. The extract and acne-cream showed 14mm and 15mm; and 13mm and 11mm against Propioni bacterium acnes and Streptococcus sp. The cream showed no irritation endpoints and it was found to be non-toxic. Thus the developed anti-acne cream can be developed as a product for the treatment of acne.