Abstract
In order to study the formation of yellow pigment in a radish root extract, a model reaction was conducted. It was found that 4-methylthio-3-butenyl isothiocyanate (4-MTBI) formed a yellow pigment in an aqueous medium by a reaction with l -ascorbic acid (l-As A) and such dihydroxyphenolic compounds as catechol, hydroquinone, dopa, dopamine and caffeic acid. No yellow colored substance was formed when the other ten isothiocyanates were employed instead of 4-MTBI. Also, thiol compounds such as l-cysteine, dithiothreitol and the reduced form of glutathione had little effect on the formation of the yellow pigment. The possibility that a water-soluble degradation product of 4-MTBI participated in the formation of the yellow pigment is suggested.
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