Formation of Malondialdehyde-Modified 2′-Deoxyguanosinyl Adduct from Metabolism of Chloral Hydrate by Mouse Liver Microsomes

Abstract

We previously reported that metabolism of chloral hydrate (CH), a widely used sedative and hypnotic, by male B6C3F1 mouse liver microsomes resulted in lipid peroxidation, producing the tumorigen malondialdehyde (MDA). Now we have found that incubation of CH in the presence of calf thymus DNA resulted in the formation of an MDA-modified DNA adduct as detected by 32P-postlabeling analysis. Similar results were obtained from incubation of trichloroacetic acid and trichloroethanol, both metabolites of CH.