Abstract
N-(Deoxyguanosin-8-yl)-2-aminopyrene and N-(deoxyadenosin-8-yl)-2-aminopyrene were formed from metabolism of 2-nitropyrene (2-NP) in vitro and in vivo. The effects of dietary restriction (DR) and age on the formation of these DNA adducts were studied. In the presence of calf thymus DNA, [G-3H]2-NP was metabolized by liver microsomes of 5-, 9-, and 12-month old male B6C3F1 mice fed ad libitum (AL microsomes) or by liver microsomes of 5-, 9-, and 12-month old male B6C3F1 mice fed a restricted diet (DR microsomes). In all cases, both adducts were detected. The total quantities formed from 5-, 9-, and 12-month AL microsomes, and 5-, 9-, and 12-month DR microsomes were 5.58±1.78, 8.79±2.32, 3.57±0.34, 1.63±0.40, 5.46±0.21, and 4.15±0.94 pmol 2-NP/mg DNA, respectively. These results suggest the effect of DR on DNA adduct formation may be age dependent. However, the ratio of dG/dA adduct formation was similar in all cases and was independent of age and caloric intake of the mice used for the microsomal preparations.
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