Abstract
Hydrogen gas production was observed to occur during ATP-driven H +/K + exchange in anaerobically grown E. coli. Neither process was found in aerobically grown cells or anaerobic cells grown on nitrate medium or when the osmotic pressure was decreased or K + removed, or finally when DCCD, arsenate or CCCP was applied. Dithiothreitol restored the process even in the presence of CCCP but not in other cases of inhibition. A model of a multienzyme transport supercomplex is proposed. The supercomplex consists of three genetically independent mechanisms: F 0F 1 H +-ATPase to provide energy, the K +-transporting Trk system as energy sink and formate-hydrogen lyase as donor of reducing equivalents. Within this supercomplex direct transduction of energy is accomplished via oxidation of 2 SH to S-S.
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