Abstract
Bacteria, isolated from lakewater and capable of growing at the expense of commercial synthetic 2-stroke lubricant oils released 2-ethylhexanol from this substrate by means of esterases. The alcohol accumulated to approximatedly 6mM in these incubations but was itself mineralized by a strain of Pseudomonas putida isolated from the same lakewater by elective culture. The initial steps in the degradative pathway of 2-ethylhexanol were catalysed by alcohol and aldehyde dehydrogenases. The resultant 2-ethylhexanoic acid was further metabolised via β-oxidation. The accumulation of intermediates in culture fluids facilitated by acrylate addition and higher isocitrate lyase activities in cell extracts, suggested that 2-ethylhexanoic acid was cleaved to two butyrate moieties which were then further metabolised to acetate. The pathway shows the organism has an interesting β-oxidation system capable of utilizing branched substrates.
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