Abstract
Extraction of water and lysozyme in mixed micellar systems of sodium bis(2-ethylhexyl) sulfosuccinate and various long chain alkyl amines were investigated. Extraction of water in the mixed micellar systems decreased rapidly with decreasing pH in the aqueous phase. The pH dependence on water extraction is affected by the structure of the amines. Extraction of lysozyme is also controlled by the formation of the reverse micelles, and does not completely occurr in the pH range in which no water is extracted into the organic phase. Lysozyme extracted into the mixed micellar systems can be successfully back-extracted with high activity yield by destruction of the micelles at acidic pH. By increasing the amine concentration, the pH values initiated by the back-extraction of lysozyme are raised, and the activity of the back-extracted lysozyme is decreased.
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