Abstract
1. 1. A highly purified sample of the sodium salt of hyaluronic acid (HA) was prepared free of contaminants from the vitreous humor. 2. 2. The HA was studied by ultracentrifugation, light scattering, streaming birefringence and electron microscopy. All of the data could be interpreted in terms of an asymmetric molecule of particle approximately 6,000 A in length which contracted to approximately one half this value in the presence of ions. 3. 3. The data indicate a polydisperse material of weight average molecular weight 1.27·10 6. 4. 4. Electron microscopy revealed thread-like particles which were sensitive to the depolymerizing action of hyaluronidase. The particles were intermediate in form between a stiff rod and a flexible coil. There were no large spheres present and the dimensions and form seen in electron microscopy were in agreement with similar values deduced by light scattering and streaming birefringence studies.
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