Forensic Conception: DNA typing of FTA Spotted Samples

Abstract

Article history: Received on: 23/06/2014 Revised on: 26/07/2014 Accepted on: 10/08/2014 Available online: 27/08/2014 Forensic labs globally face the same problem growing need to process a greater number and wider variety of samples for DNA analysis.DNA purification step has been thought to be essential for typing of STR DNA. However, this process is time-consuming, and there is a risk of unexpected cross-contamination during purification. There is a need, for each sample type, to develop new methodology that is not only faster but also more reliable than past procedures. FTA technologies provides use most reliable method for DNA extraction.FTA is a chemical treatment of paper, unique to Whatman Bioscience, and is used for the stabilization and storage of biological samples. The FTA matrix is chemically treated with proprietary reagents that lyse cells upon contact. FTA cards protect nucleic acids from nucleases, oxidation, UV damage and microbial and fungal attack. In forensic laboratory there is a need of such a method by which DNA can be extracted by simple method in less time. Here, the method is modified for semen and saliva samples for extraction of DNA on FTA classic cards for forensic use. All the samples were collected only on FTA Classic cards. After DNA extraction, amplification of DNA was carried out then the amplified DNA was placed into DNA sequencer for STR profiling. STR peaks were obtained from the all biological samples such as Blood, Saliva and Semen in our study thus there is no need of other kits available for collection of samples. On the basis of results, it is considered that modified procedure using an FTA card can be applicable to routine work.