Forensic application of autosomal STR analysis in Lithuanian population

Abstract

The data presents the first comprehensive autosomal STR analysis in the Lithuanian population for the purpose to compile an autosomal STR DNA database of natives from Lithuania, evaluate autosomal STR diversity, introduce population reference data for forensic and population genetic issues. Autosomal STR data were collected from the blood samples of 300 unrelated individuals distributed throughout the country. The amplification of 15 autosomal STRs was performed in one multiplex PCR by the use of AmpFlSTR ® Identifiler ® PCR Amplification Kit for human identity and parentage testing. Statistical analyses were carried out to determine the basic parameters of population genetics and forensic efficiency for 15 autosomal STRs. The results indicated that autosomal STR profiles enable to achieve high-resolution essential for forensic DNA casework.