Abstract
A method for the determination of 11 heterocyclic amines (HAs) in meat extracts by high performance liquid chromatography with electrochemical detection is proposed. The chromatographic method is optimized to obtain both polar and less polar amines in the same chromatographic run, which is a difficult task when using electrochemical detection. The extraction of the meat extracts is carried out using a focused microwave system, a technique applied for first‐time in the extraction of these analytes. The microwave extraction process takes only 5 minutes, an improvement on the extraction times for methods commonly applied to heterocyclic amines in food samples. This screening method allows us to determine HAs in meat extracts with average recoveries of 81.2%, 76.4%, 66.7%, 60.3%, and 63.8%, for granulated meat extract, non‐granulated meat extract, chicken extract, and soup cubes (brands A and B), respectively. The proposed method has an intermediate precision ranging from 6.1 to 12.8%, and detection limits between 0.16 and 2.68 ng.
Published Version
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