Fmoc-based synthesis of glycolate ester peptides for the assembly of de novo designed multimeric proteins using subtiligase

Abstract

Listen

Translate article

An automated method utilizing Fmoc-protected amino acids has been developed for the synthesis of glycolate ester peptides as substrates for subtiligase. As a test of this methodology, peptide esters containing α-helical sequences that specify the association into 3- and 4- helix bundles were synthesized, and used to explore the suitability of subtiligase as a tool for the generation of covalently linked bundles. The feasibility of creating de novo designed, single-chain or cyclic structures from simple, readily-synthesized modules has been demonstrated.